Ultra-Pure Ligated sgRNA (LgRNA) for Precise Gene Editing

GeneLancet Biosciences’ platform addresses key challenges in CRISPR gene editing. Today, CRISPR-Cas9 gene editing utilizes single guide RNA (sgRNA). While sgRNA is effective in achieving consistent editing results, synthetic sgRNA suffers from low overall product purity (<50% LC-MS purity), which results in reduced editing efficiency and specificity. Fully-functional, truncated, lot-dependent impurities in sgRNA pose catastrophic risks to patients.

The crRNA in our ligated single-molecule guide RNA (LgRNA) is 32 nt long, and its high purity is readily accessible by routine purifications before ligation, which selects the full-length product of tracrRNA. All GeneLancet’s LgRNA products contain undetectable truncated spacer sequences and are ultra-pure (up to 99% by LC/MS) with very low levels of biologically active impurities. Our cutting-edge LgRNA technology in Doylestown, Pennsylvania, ensures these quality standards are consistently met to advance precise gene editing applications.

  • GeneLancet’s LgRNA is highly pure, resulting in very high specificity
  • LgRNA has high editing efficiency
  • Production of LgRNA is robust, scalable, and highly cost-effective
  • GeneLancet’s LgRNA platform is proprietary and intellectually distinct

Advancing Precision Medicine With Smarter RNA Tools

At GeneLancet Biosciences, we are constantly innovating to push the boundaries of genetic science. Our New-generation Guide RNA (LgRNA) Platform is a next-level solution designed to improve the specificity and efficiency of genome editing technologies. With its optimized structure and enhanced targeting ability, LgRNA plays a vital role in unlocking more effective, safer therapeutic interventions.

Why LgRNA Technology Changes the Game

Our New-generation Guide RNA (LgRNA) Platform offers a transformative approach for next-gen therapeutics by enhancing the precision and function of CRISPR-based tools. Designed with therapeutic applications in mind, this platform empowers researchers and biotech developers with greater flexibility, fewer off-target effects, and more robust outcomes. By integrating LgRNA into our solutions, GeneLancet Biosciences is helping redefine how gene-targeting therapies are developed and delivered.

  • Increases gene-editing accuracy, reducing risks tied to off-target activity
  • Improves the stability and efficiency of RNA molecules in complex therapeutic environments
  • Enables more flexible designs for targeting a wider range of genetic mutations
  • Supports scalable manufacturing, making it ideal for both early research and clinical deployment
  • Unlocks potential for advanced treatments across rare diseases, cancer, and inherited disorders

GeneLancet Biosciences remains committed to leveraging cutting-edge platforms like LgRNA to bring forward the next generation of safe and targeted genetic therapies. With a strong focus on innovation and patient outcomes, our team is shaping the future of precision medicine through reliable and intelligent RNA solutions that drive real therapeutic impact.

Frequently Asked Questions

What is a next-gen guide RNA platform?
A next-generation guide RNA platform uses advanced molecular tools to direct gene editing systems more precisely. It helps improve accuracy and reduce unwanted effects. We use this type of RNA in our research and therapeutic development work.
How is LgRNA different from regular guide RNA?
LgRNA is longer than traditional guide RNAs, which can improve targeting and stability. This added length can make gene editing more specific, helping us develop treatments with fewer risks.
Is LgRNA safe to use in therapies?
Safety testing is a major part of developing any therapeutic. We follow strict research guidelines and work closely with regulators to evaluate LgRNA-based treatments before they move into clinical use.
Is using guide RNA safe for patients?
Safety is a top priority. The platform is tested carefully to minimize risks like unwanted genetic changes, and ongoing studies help confirm its safety for potential treatments.
What kinds of conditions could be treated with guide RNA technology?
This technology shows promise for treating genetic disorders, certain cancers, and viral infections by correcting or disabling harmful genes.

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